Answer:
Retrovirus
Explanation:
Reverse transcriptase is a type of enzyme which is present in many RNA based viruses such as retrovirus that are found in humans. Reterovirus uses reverse transcriptase enzyme in order to change their single stranded DNA into double stranded DNA. The process of such type of conversion is called reverse transcription. In viruses such as reterovirus, reverse transcriptase allows the virus to insert its DNA to the host cell's DNA, which helps the virus to produce more viruses.
Animals cells have a mitochondria, plant cells have a vacuole, a chloroplast and a cell wall for protection.
Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
1. Testing only one variable
A controlled variable means its the one that stays the same. In a controlled experiment, it means there is only one manipulated, (changed) variable.
