1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
matrenka [14]
3 years ago
12

You are given a flask with standard liquid media containing a population of S. pombe cells that have a temperature sensitive mut

ation of a gene that causes complete loss of function of the gene product at the restrictive temperature. The flask had been incubating at the permissive temperature in the dark before it was given to you, and the cells in the flask were replicating asynchronously with respect to the stage of the cell cycle. The mutated gene in this strain of yeast encodes an essential component of a UV-mediated DNA damage checkpoint. Which FACS plot (see attached figure) best represents how cells in this flask would appear following growth for 30 min at the restrictive temperature ? (Note, assume that FACS plot #1 shows analysis of a population of S. pombe replicating asynchronously (i.e. each cell within a population is at a random stage of the cell cycle) in culture. In the rich media assume the yeast have a cell cycle time of 100 min, with G1=50 min; S = 25 min; G2 = 15 min and M = 10 min).a. #2 b. #3 c. #4 d. #5 e. #6
Biology
1 answer:
Ne4ueva [31]3 years ago
4 0

Answer:

The best graphic that represent the growth of the cells is the plot #2

Explanation:

Using flow cytometry, a cell cycle analysis study uses a dye for DNA binding, and it is fluorescently labeled to allow stoichiometry of DNA to bind to DNA content.

During the phases, the DNA content will be:

G0 / G1 phase = 2n (diploid).

Phase S = 2n (duplication of DNA content)

G2 / M phase = 4n (content of tetraploid DNA 4n)

When cells are growing, a mutation will occur in the gene. This would lead to the cell cycle being unlikely to stop due to the checkpoint gene mutation, causing it to be unable to detect any damage. the cell cycle continues normally.

In exercise, cells that were in the G1 phase will reach the S phase. Therefore, the peak of that phase will be increased, and the peak of the G1 phase will decrease. The cells that were in the S phase will go to the G2 / M phase, which will increase the peak.

You might be interested in
What is the biogeochemical cycle that describes the movement of oxygen within and between its three main reservoirs?
Komok [63]

Oxygen Cycle. Hope this helps

8 0
3 years ago
Question 8(Multiple Choice Worth 4 points)
solong [7]

Answer:

D/ Kilograms per cubic-centimeter

7 0
3 years ago
Read 2 more answers
Helpppp , I’ll give you 25 points and a brainy .
neonofarm [45]
A Respiration by animals CO2 is being added.
B trees burning same
C tree leaves removed
D oil added
8 0
3 years ago
Which of the following is a testable hypothesis?
beks73 [17]

The answer is B.

If you put one plant in wet soil, and one plant in dry soil, you can prove or disprove your hypothesis as the plants grow.

8 0
3 years ago
What property of the DNA molecule explains the necessity for Okazaki fragments? DNA is antiparallel. DNA is a linear molecule. D
Arada [10]

Answer:DNA is antiparallel.

Explanation: DNA is a double stranded helix in which the two strands are antiparallel. Being antiparallel means that as one strand runs from 5'->3' direction, the other strand runs from 3'->5' direction. During DNA each of the two strands serves as a template for a new complementary strand. The synthesis of a new DNA strand is always in the 5'->3' direction, therefore one strand is synthesized continuously in the direction of the replication fork while the other strand is synthesized discontinuously in the direction opposite to the replication fork in short fragments called the Okazaki fragments. The strand that is synthesized continuously is called the leading strand while the strand that is synthesized discontinuously in Okazaki fragments is called the lagging strand.

3 0
3 years ago
Other questions:
  • Why is the solid earth layered?
    15·1 answer
  • Which of the following is the best example of an organism maintaining homeostasis
    8·2 answers
  • What are some things that should be checked before blood is transfused
    12·1 answer
  • 16. the process of making an inference,
    9·1 answer
  • HELP ME PLS!!! Which determines carrying capacity? a) logistic factors b) exponential growth c) population growth d) limiting fa
    13·2 answers
  • Some fungi are able to reproduce both sexually and asexually. Which of the following describes a consequence that would most lik
    14·1 answer
  • Complete the following sentence.
    11·2 answers
  • I need to know the answer to the question
    12·2 answers
  • No links and i know this isn't actually biology
    15·1 answer
  • Hello please give answers
    11·2 answers
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!