Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Bioremediation is the process of using living organisms to detoxify a location. Microbes are used to clean up contaminated soil and groundwater. The organisms that are used in this process are: bacteria, fungi, archaea. For example, b<span>acteria are introduced to the area of the spill where they break down the hydrocarbons of the oil into carbon dioxide;</span>..
Answer:
I believe it is A.
Explanation:
Im not 100% sure though so I'm so sorry if it's wrong.
This is true. There are a few websites online that talk about how it happens, I suggest looking them up.
Goiters are often produced in the Thyroid. Goiters is an enlarged thyroid gland. Goiters are believed to be painless, but Goiters make it difficult for the victim to breathe and swallow food. Goiters are caused by lack of iodine in one's diet. Treatment of Goiters depends on its size, some Goiters go away without any treatment while some goiters are treated through surgery.
