Answer: What substances dissolve in ocean water.
This is the only question that can be answered through scientific investigation because it is the only question that can be tested. The other questions cannot be answered through scientific investigation because they based on opinions which are more abstract.
Answer:
Less urine production.
Explanation:
The urine production rate in the Pedialyte group is lower as compare to that of the water group because the presence of K, Cl-, and Na ions. The precise combination of electrolytes and sugars in Pedialyte helps in restoring fluid levels and the nutrients in the body that are lost through sweating, urinating, or vomiting and diarrhea. We know that the presence of sodium ions regulation water and electrolyte balance in the body so there is less urine produced by taking Pedialyte group.
Simple version:
First, the section with the desirable gene must be identified. Assuming that has already happened, the section of DNA must be excised from the original genome using restriction enzymes, which recognize certain DNA sequences and snip DNA at those sites. DNA ligase is used to "glue" these ends back together. The DNA is inserted into a plasmid (also with restriction enzymes), which would usually contain antibiotic-resistance genes (so they survive in an environment containing the antibiotic, which would also help show if the bacteria have been successfully transformed).
Then comes the actual transformation process. The bacteria to be transformed are mixed with calcium chloride (which causes the bacteria to be more receptive to the plasmids) and then mixed with the plasmids. The bacterial cells are subjected to a heat shock (the solution is heated and rapidly cooled, e.g. by placing the mixture in a hot water bath and quickly transferred to ice) so they will take up the plasmid (since the temperature change makes the membrane more permeable). The bacteria are placed on a growth medium containing the antibiotic they're resistant to. Only those successfully transformed would survive.
