Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Hello there,
1.) According to the biomolecular data, we COULD infer that <span>humans are more closely related to chimps than any other apes.
2.) </span>Based on the cladogram, if moths undergo complete metamorphosis, we would MOST LIKELY infer that <span>butterflies and flies do as well.
3.) </span>According to the cladogram, which of these were the direct ancestors of the Kingdom Fungi? <span>Protista
4.) </span>Trey is looking at a cell under the microscope. It is attached to many other cells, and it has a nucleus and membrane bound organelles. It has no cell wall. In which kingdom should this organism be classified? <span>Fungi
5.) </span>A review of the cladogram shows a common ancestor for these four types of vertebrates. Which statement BEST explains the genetics represented by the cladogram? <span>Lizards and snakes are more genetically alike than lizards and birds.
Hope this helps
-HotTwizzlers :D</span>
Answer: Peripheral System
Explanation:
The best answer to go with is b you’re welcome
