Answer: During electron transport, energy is used to pump hydrogen ions across the mitochondrial inner membrane, from the matrix into the intermembrane space. A chemiosmotic gradient causes hydrogen ions to flow back across the mitochondrial membrane into the matrix, through ATP synthase, producing ATP.
<span>The question makes it seem that you looked through both types of microscopes at specific samples? As for how they would differ, a light microscope's level of magnification is limited by the physical characteristics of light and therefore can only see objects as small as organelles inside of a cell. A scanning electron microscope, however, does not use light, it uses a beam of electrons to visualize the sample. Electrons are much smaller than the light beam and are able to image much smaller objects, such as molecules and atoms.</span>
