1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
sweet [91]
3 years ago
11

A protein sample has been treated differently before being loaded onto the gel for SDS-PAGE. The first aliquot of the sample is

treated with SDS. The second aliquot of the sample is treated with SDS and a reducing agent. If there is no difference in the number of bands when comparing the results of the two experiments, the results most likely indicate...
Select one:
a. experimental contamination
b. that the proteins' non-covalent forces were additively stronger than the force of the disulfide bond(s)
c. that the protein was not denatured fully
d. that the protein being studied contain disulfide bonds in its quaternary structure
e. that the protein being studied does not contain disulfide bonds in its quaternary structure
Biology
2 answers:
Anettt [7]3 years ago
5 0

Answer:

The correct answer is option e. "that the protein being studied does not contain disulfide bonds in its quaternary structure".

Explanation:

Reducing agents, such as  ß-mercaptoethanol (BME) or dithiothritol (DTT), are elements or compounds that break down disulfide bonds. These agents reduce the disulfide bonds and prevent the oxidation of the thiol groups of the cysteine residues that form the disulfide bonds. If one protein that was not treated with a reducing agent have the same electrophoretic behavior than a protein that was treated with a reducing agent in a SDS-PAGE experiment, then the results most likely indicate that the protein being studied does not contain disulfide bonds in its quaternary structure.

MrRa [10]3 years ago
5 0

Answer:

e. that the protein being studied does not contain disulfide bonds in its Quaternary structure

Explanation:

SDS-PAGE is an analytical method that is used to separate charged molecules such as proteins.

In the above experiment, the protein sample was treated with SDS. SDS disrupts the hydrogen bonding in proteins making it linear in conformation.

It also provides a negative charge enabling the proteins be separated according to size only.

On the other hand, reducing agents serve to break disulfide bonding in the proteins.

Therefore, the protein being studied in the experiment lacked disulfide bonds hence there was no difference in bands.

You might be interested in
Mitochondria and chloroplasts
Grace [21]

Answer:

C. prokaryotic cells

Definitions

The Mitochondrion is an organelle in the cytoplasm of cells that functions in energy production.

Chloroplast is usually defined as a plastic containing chlorophyll.

Chlorophyll is the green coloring matter of leaves and plants, essential to the production in a bluish-black form, C55H72MgN405 (chlorophyll a), and a dark-green form, C55H70MgN4O6 (chlorophyll b).  

A root cell is one of the maestro cells in the human organic structure that has possible ability of turning into any of the more than the organic structure's 200 cell types.  

Prokaryotes is any cellular organism that has no nuclear membrane, no organelles in the cytoplasm except ribosomes, and has its genetic material in the form of single continuous strands forming coils or loops, characteristic of all organisms in the kingdom Monera, as the bacteria and blue-green algae.

<h2>What are the similarities and differences between prokaryotes, mitochondria, and chloroplasts?</h2>

Both mitochondria and heterotrophic prokaryotes, as well as chloroplasts and autotrophic prokaryotes, have a genetic structure (DNA with a circular double helix shape and the genes themselves), as well as comparable ribosome types. In reality, the endosymbiotic idea is based on these parallels.

5 0
2 years ago
Vitamins help enzymes function as _____. A.proteins <br> B.coenzymes <br> C. minerals <br> D.fats
vekshin1
Vitamins help them function as fats.
4 0
3 years ago
Why are enzymes important to cells? they help bring about chemical reactions. they store large quantities of energy. they provid
Paladinen [302]
They help bring about chemical reactions
8 0
4 years ago
Read 2 more answers
Why are food preservatives add
irina [24]
To preserve and keep them fresh
3 0
3 years ago
Read 2 more answers
Electrons are the electrons in the outer energy
tamaranim1 [39]

Answer:

Valence

Explanation:

In an atom, the electrons at the outermost energy level is called as valence electrons. It is the valence electrons which participates and interacts with the other atoms and form bonds. The valence electrons of an atoms are important as they depict the chemical properties of an element.

Thus the valence electrons resides the outermost shell and surrounds the atomic nucleus.

6 0
3 years ago
Other questions:
  • Why are the interactions of hormones and tissues considered to be feedback mechanisms
    7·1 answer
  • How do molecular genetics add to our understanding of genes?
    12·1 answer
  • A scientist is trying to determine the relationship between clams, snails, and squid. When she generates a molecular clock, she
    9·2 answers
  • How are the neutral theory of molecular evolution and the molecular clock related?
    14·1 answer
  • A quantity of a gas has an absolute pressure of 400 kPa and an absolute temperature of 110 degrees kelvin. When the temperature
    13·2 answers
  • In some cases, recombinant DNA must be cloned before it can be inserted into a host. Which vectors can be used to clone recombin
    15·2 answers
  • 1. Using the graph, describe the events that occur in the body during the first year of
    14·1 answer
  • A single hydra can produce offspring by growing a new individual from any portion of its body. What is the reproduction method o
    11·2 answers
  • 18
    9·1 answer
  • Match The Animals With Their Characteristics.<br>​
    14·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!