There are four symptoms of addiction namely:
Obsession
Negative consequences
Lack of control
Denial
In this patient, he denies that his behavior is self-destructive; even with the concrete evidence that he is being self-destructive (i.e. plays all day even with work to do). There are two descriptions of denial wherein; (1) he denies that what he does cannot be controlled and (2) he denies that what he does leaves a negative impact in his life. This patient falls to the latter description of denial.
Answer: To eliminate bacteria that failed to take in the plasmid.
Explanation:
Research shows that plasmid DNA is only taken in by small percentage of bacteria in a large population.
Therefore, the need of growing transformed bacteria in the presence of ampicillin is very important. The main reason of this process is to kill off or eliminate bacteria that have failed to take in the plasmid, due to their possession of recombinant DNA plasmid pARA-R (an ampicillin resistance gene), that allows them to survive.
However, after completion of the aforementioned procedure, the bacteria that have been transformed will be able to take in the plasmid.
I’m guessing it will be thunderstorm Bc cumulonimbus clouds are very heavy
What can I do to assist??
