<h3>A process by which the contents of a cell vacuole are released to the exterior through fusion of the vacuole membrane with the cell membrane is Exocytosis.</h3>
The process of physical change in state, that occurs when water vapor or gas converts into a liquid is condensation. According to the kinetic molecular theory, the particles lose kinetic energy and slow down and thus become more arranged and have a definite shape but an indefinite volume, a liquid.
Uses natural pressure
Has groundwater stuck between impermeable layers
are the statements which describe the attributes of an Artesian well.
<u>Explanation</u>:
- Artesian well, well from which water is pumped under natural pressure without pumping. It is dug or dug wherever a soft dip, porous rock layer obtains water along its ridge at a point higher than the rate of the ground surface at the well site
- .First, artesian groundwater is the same as any other groundwater type; the only distinction is how it reaches the surface.
- An artesian well is simply a well that doesn't involve a pump to get water to the surface it happens when there is sufficient pressure in the aquifer. This gives rise to positive pressure.
Answer:
Use a step wise process
Explanation:
In order to find the presence of the recombinant protein:
- Culture the cells that you believe harbors the plasmid for the recombination protein (+) and also cells that do not (-). This is your control.
- Extract the plasmid from both strains.
- Observe the plasmid map and the area of your insert. Use this to select the restriction enzymes at the beginning and the end of the YFG gene. You can also use a restriction site inside the gene but not in the plasmid for better control.
- Perform the restriction enzyme digest on both (+) and (-) according to your protocol and separate using gel electrophoresis.
- Observe the separate bands that you see. In the (+) there should be a band that is the size of your insert and a larger band that is the size of your plasmid control band and in the (-) there should be a single band that is the size of the plasmid but non the size of the insert.