Forward primer: 5'-AGTCTACTCGTAACCGGTTACC-3' Reverse primer: 5'-TAAGGCATCATGGTAACCGGTT-3' The two primers above have the melting
temperatures between 55C-80C. What is the reason that these two primers will not bind effectively to the region of DNA that we want to amplify?a. The length of the two primers is between 20-30 base pairs long. The length is too short and not very specific.b. The 3'-end of the reverse primer (3'-TTGGCCAATGG---5') is complementary to the forward primer (5'---AACCGGTTACC-3') and thus reduces the ability of the primers to bind to the targeted DNA sequence.c. The 3'-end of the forward primer is complementary to the 5'-end of the reverse primer and thus reduces the ability of the primers to bind to the targeted DNA sequence.d. The melting temperature range of the primers is similar to the DNA.e. Guanine and Cytosine make up 60% of the total base pairs.
The 3'-end of the reverse primer (3'-TTGGCCAATGG---5') is complementary to the forward primer (5'---AACCGGTTACC-3') and thus reduces the ability of the primers to bind to the targeted DNA sequence. There is possibility of hairpin loop formation or primer dimmers formation
