B. Histones is the correct answer.
George Monbiot is spearheading the Rewilding Movement. The environmentalist and writer is prominent in the platform. He was featured in TED Talk and talked about a large scale conservation approach aiming to restore the lost habitats. Here is an excerpt of his speech where he discusses the lost megafuna:
"What megafauna, you say? Well, every continent had one, apart from Antarctica. When Trafalgar Square in London was excavated, the river gravels there were found to be stuffed with the bones of hippopotamus, rhinos, elephants, hyenas, lions. Yes, ladies and gentlemen, there were lions in Trafalgar Square long before Nelson's Column was built. All these species lived here in the last interglacial period, when temperatures were pretty similar to our own. It's not climate, largely, which has got rid of the world's megafaunas. It's pressure from the human population hunting and destroying their habitats which has done so."
Answer: To eliminate bacteria that failed to take in the plasmid.
Explanation:
Research shows that plasmid DNA is only taken in by small percentage of bacteria in a large population.
Therefore, the need of growing transformed bacteria in the presence of ampicillin is very important. The main reason of this process is to kill off or eliminate bacteria that have failed to take in the plasmid, due to their possession of recombinant DNA plasmid pARA-R (an ampicillin resistance gene), that allows them to survive.
However, after completion of the aforementioned procedure, the bacteria that have been transformed will be able to take in the plasmid.
1. Smoking
2. Weight
3. Alcohol
4. Drugs
5. Depression
