When you start the PCR amplification reaction, you have a template which has sequences where the primers will anneal. The forward primer anneals in the sense strand and the reverse one in the complementary sequence (see attached figure).
After the first PCR cycle, two types of fragments are obtained. First those which come from tha 5'-3' sequence, that are 3'-5' and will anneal the reverse primer in the following cycle. This fragments have the correct 3' end, but the 5' includes part of the template that doesn't correspond to our target (see attached figure)
Second, those fragments that come from the 3'-5' sequence, that are 5'-3' and will anneal the forward primer. This fragments also have the correct 3' end, but the 5' includes part of the template that doesn't correspond to our target (see attached figure).
Now, during the second cycle, are produced the first fragments that have the precise length of the sequence that we want to amplify (see attached figure).
So, it's not until the third cycle that the reaction starts amplifying exactly the sequence of desired length.
Answer:
B cell membranes protect a cell,and cell walls do not
Desertification extends beyond the expansion of existing deserts to include land degradation due to human activity in drylands. Land degradation destroys or reduces the productivity of the soil, and is caused by excessive and unsustainable activities such as deforestation, overgrazing, poor land management, soil salinization, and soil erosion.
Sure hope this helps you :D
C. Birds have the same arrangement of bases as flowers
Answer:
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Explanation:
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