<span>The role of a restriction enzyme is essentially used to cut a single gene from a larger piece of DNA. It has a very important role to play in experiments investigating cloning, and in the construction of particular DNA molecules.</span>
        
                    
             
        
        
        
Answer:
Lung function tests (also called pulmonary function tests) include a variety of tests that check how well the lungs work. The most basic test is spirometry. This test measures the amount of air the lungs can hold. The test also measures how forcefully one can empty air from the lungs.
 
        
             
        
        
        
<span>has a 50 percent chance of passing the mutated gene to each child. The chance that a child will not inherit the mutated gene is also 50 percent. </span>
        
                    
             
        
        
        
Hi!
The answer is B. chloroplast because prokaryotic cells don't have contractile vacuoles, DNA, or a nucleus. 
Hope this helps!  :))<span />