To identify the molecule that accepts , Calvin and Benson manipulated the carbon-fixation cycle by either cutting off or cutting off light from cultures of photosynthetic algae. They then measured the concentrations of various metabolites immediately following the manipulation. The acceptor concentration would increase when the is cut off, but decrease when the light is cut off.
The correct option is d.
The process through which living things absorb inorganic carbon from the atmosphere and transform it into organic molecules is known as carbon fixation. These substances are employed as chemical energy storage. For life to continue, it is a necessary process.
Calvin and Benson disrupted the carbon-fixation cycle by either removing or removing light from cultures of photosynthetic algae in order to find the molecule that takes . Following the alteration, they evaluated the concentrations of several metabolites.
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Answer: The bacteria transformed with this particular plasmid will form white colonies on the plates containing ampicillin and Xgal.
Explanation: The lacZ gene produces an enzyme called β-galactosidase which is responsible for the breakdown of lactose into glucose and galactose. The lacZ gene is one of the three genes (the other two being lacA and lacY) of the lac operon which is responsible for the transport and mechanism of lactose in E. coli and many other bacteria.
In recombinant DNA technology, when a plasmid is to be used to transform a host cell, such markers are used to help screen the transformed cells from the ones that have not taken up the plasmid. Xgal present in the plates is an artificial substrate which is hydrolyzed by
β-galactosidase into 5-bromo-4-chloro-indoxyl which will dimerize and oxidise into 5,5'-dibromo-4,4'dichloro-indigo. This is a blue pigment which will give blue color to the bacterial cells. Introducing a DNA fragment in this lacZ gene will make it non-functional so it will not be able to produce the enzyme.
Therefore, when a bacterial cell is transformed with a plasmid containing ampicillin resistance gene and a DNA fragment introduced in the lacZ gene and then grown on plates containing ampicillin and Xgal, white colored colonies will appear. The white colonies will show the bacterial cells that have successfully taken up the plasmid with the DNA fragment incorporated in the lacZ gene as this will render the gene non-functional and will not produce β-galactosidase which will breakdown Xgal to give blue colonies. Since the plates contain ampicillin, only the bacterial cells that have been successfully transformed with the plasmid ( the ones that have the DNA fragment and the ones without it) will grow as the ampicillin resistance will give them resistance against ampicillin in the plates. The bacterial cells that have not taken up the plasmid will not be resistant to ampicillin and will not form colonies on the plate.
This is called blue-white screening which is used to identify successfully transformed host cells. A picture of this is given in the attachment, taken from the following website:
https://www.mun.ca/biology/scarr/Blue_&_White_Colonies.html
The free nerve endings
can detect pain, temperature, itch and hair movement. Merkel's disks can detect pressure, position and static touch features. Pacinian corpuscles are sensitive to vibration while Meissner's corpuscles are sensitive to light and touch.
A characteristic of carbon is that it is highly reactive and can form unstable bonds with any available atom.
The skeletal muscles have striations to do their sacromere anatomy
sacromere which are formed by actin and myosin stacking
sarcomere is the functional unit of muscles and shows striated appearance due to thick and thin filaments
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