Your heart is both fragile and strong. It is strong in love, but it is easily broken. Its hard to break up with them because your love for them, your bond to them, is strong and they mean the world to you. Its like you're taking your own world from you. You're ripping away the part of you that you love the most; Them. The worst part about it though, Is that you're the one who is making the choice and you know it will kill them just as much as you. However, If it is absolutely best for both of you, It must be done. Im sorry you're going through this. If you need someone to talk to, you can talk to me if you need to. Good luck!
You measure the paper you are using to draw the map on and you measure the size of the country you are putting on your paper, then you can calculate the measurements and divide by how much states can fit in on your paper.
Answer:Objective: To determine the combined effect of oxygen level and glucose concentration on cell viability, ATP production, and matrix synthesis of temporomandibular joint (TMJ) disc cells.
Design: TMJ disc cells were isolated from pigs aged 6-8 months and cultured in a monolayer. Cell cultures were preconditioned for 48 h with 0, 1.5, 5, or 25 mM glucose DMEM under 1%, 5%, 10%, or 21% O2 level, respectively. The cell viability was measured using the WST-1 assay. ATP production was determined using the Luciferin-Luciferase assay. Collagen and proteoglycan synthesis were determined by measuring the incorporation of [2, 3-(3)H] proline and [(35)S] sulfate into the cells, respectively.
Results: TMJ disc cell viability significantly decreased (P < 0.0001) without glucose. With glucose present, decreased oxygen levels significantly increased viability (P < 0.0001), while a decrease in glucose concentration significantly decreased viability (P < 0.0001). With glucose present, decreasing oxygen levels significantly reduced ATP production (P < 0.0001) and matrix synthesis (P < 0.0001). A decreased glucose concentration significantly decreased collagen synthesis (P < 0.0001). The interaction between glucose and oxygen was significant in regards to cell viability (P < 0.0001), ATP production (P = 0.00015), and collagen (P = 0.0002) and proteoglycan synthesis (P < 0.0001).
Conclusions: Although both glucose and oxygen are important, glucose is the limiting nutrient for TMJ disc cell survival. At low oxygen levels, the production of ATP, collagen, and proteoglycan are severely inhibited. These results suggest that steeper nutrient gradients may exist in the TMJ disc and it may be vulnerable to pathological events that impede nutrient supply.
Explanation: The evaluations a Mainly in the oganic responses
