Question

What kind of evidence could be used to determine the percentage of the genome that is transcribed? Match the words in the left column to the appropriate blanks in the sentences on the righ

Answer 1

The question was incomplete as the sentences and the words are missing, however, the correct sentences are as-

One approach: ......... could generate the information needed. Add ...... precursor to a culture of growing cells to label all ........ being synthesized in vivo. Then test binding of bulk labelled RNA to ......... representing most of the DNA species in the genome of the organism. The percentage of ........ species that bind ......... species will be a measure of the percentage of genome being transcribed. Another approach: ........ of the RNA species in a total RNA extract. ............ is needed to be carried out later to detect all DNA sequences present.

Answer:

1. Microarray data

2. Fluoroscent RNA

3. RNAs

4. A microarray

5. DNA

6. Fluoroscent RNA

7. Reverse Transcription

8. Deep sequencing

Explanation:

Microarray data, reverse transcription, and deep sequencing are the approaches can be used to determine the percentage of the genome that is transcribed.

One approach:

Microarray data could generate the information needed.

Add Fluorescent RNA precursor to a culture of growing cells to label all RNA's being synthesized in vivo.

Then test binding of bulk labelled RNA to A microarray representing most of the DNA species in the genome of the organism.

The percentage of DNA species that bind Fluorescent RNA species will be a measure of the percentage of the genome being transcribed.

Another approach: Reverse transcription of the RNA species in a total RNA extract.

Deep sequencing is needed to be carried out later to detect all DNA sequences present.