Hi
Below are five major steps of DNA or gene cloning:
1: You chose the gene or specific piece of DNA you need to clone and cut the gene with restriction enzymes from the source organism.
2: You need to choose a vector for the process of cloning and you will cut the vector with same restriction enzymes through which you have cut your target DNA sequence to be inserted into the vector.
3: You place the target gene into the vector and join or seal the gene with vector by using an enzyme called DNA ligase.
4: You introduce the vector with your target gene into a suitable host organism such as yeast or bacteria through the process of Transformation. In this process host organism takes up the vector containing your target gene and starts replicating the target DNA along with their own DNA and thus creating millions of copies of target gene .
5: In the last step, the DNA or target gene is isolated from host organism and purified and is ready to be used since its quantity has been enormously increased through the process of cloning.
The cloning is also called as recombinant DNA technology and is the main process that is being used in the production of insulin for diabetes patients. You can see below image for better understanding.
Hope it help!
Answer:
The correct answer is - d) all choices are correct.
Explanation:
Cattles remember the behaviour of human how they have treated them in the experiment and they also become less fearful of their handlers due to their gentle treatment.
Cattles develop memory and have a bond with their handlers and remember them and recognize them very well. In this experiment, it is found that animals also create an emotional bond with the human treat them well and gentle.
Living things breath, feel, and smell.
Most living things have those characteristics.
I’m not 100% sure, but I hope I helped a bit.
Answer:
cell wall and chloroplasts
Explanation:
its B!! :) cell wall in plant cells, cell membrane in animal cells.
Answer:
The correct answer: C) allows the behavior of microtubules called dynamic instability.
Explanation:
Microtubules are structures that are part of the cytoskeleton and help provide structure and shape to eukaryotic cells, besides assisting in the transport of organelles and vesicles.
Microtubules are formed by the polymerization of a dimer of two tubulins.
Microtubules have the particularity of being able to easily assemble and disassemble, which enlarges and shrinks the microtubule respectively. This property of the microtubules is important to their functions and is called dynamic instability.
In order to achieve dynamic instability, tubulin dimers can bind two molecules of GTP - for polymerization to happen, one of the GTP molecules is hydrolyzed to catalyze the reaction, thus becoming GDP. In the same matter, the energy generated from the depolymerization with turn the GDP into GTP.
