Question

E. coli cells grown on 15N medium are transferred to 14N medium and allowed to grow for two more generations (two rounds of DNA replication). DNA extracted from these cells is centrifuged. What density distribution of DNA would you expect in this experiment?
a. one high-density and one low-density band
b. one intermediate-density band
c. one high-density and one intermediate-density band
d. one low-density and one intermediate-density band

Answer 1

Answer:

Option (d).

Explanation:

DNA is the genetic material of all the organism except some viruses. DNA is composed of nitrogenous base, deoxyribose pentose sugar and the phosphate group.

DNA replicatese by semi-conservative mode of replication in which one the daughter DNA consists of one newly synthesized strand and one parental strand. This mode of replication was explained by Messelson and Sthal experiment. The cells from 15N medium are transferred to 14N medium the band of intermediate density was observed. The daughter DNA consists of one 15N DNA strand and one 14N DNA strnd. This strand undergo replication in the 14N medium. This time one low density DNA band ( contains 14N - 14N DNA strand) and one intermediate density band ( 14N-15N DNA).

Thus, the correct answer is option (d).