Answer: A) The resident populations are more likely affected by these threats because of their proximity to shore.
Explanation:
All of these threats are higher in coastal regions.
As contaminants usually reach the ocean through rivers or because of coastal cities, the highest degree of pollution is there. All chemicals will accumulate in this region first, before they disperse into the offshore region. This is also caused by the fact that the coastal waters have a lower volume and surface area than the offshore which results in a higher degree of contamination.
Bigger, the universe is expanding.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Body temperature, Environmental temperature, Body mass, Reproductive condition, Activity, Feeding, Body condition, Season. All of these would help increase it. Hope this helps. :)
A.Transmission electron microscopes are a versatile tool for many fields, including medicine, biology, nanotechnology, metallurgy, forensics, electronics, material science, and much more. A biologist might use a TEM to look at the internal structure of a cell.
b. Industries including microelectronics, semiconductors, medical devices, general manufacturing, insurance and litigation support, and food processing, all use scanning electron microscopy as a way to examine the surface composition of components and products.
c.Brightfield Microscope is used in several fields, from basic biology to understanding cell structures in cell Biology, Microbiology, Bacteriology to visualizing parasitic organisms in Parasitology. Most of the specimens to viewed are stained using special staining to enable visualization.
d.A dissecting microscope is used to view three-dimensional objects and larger specimens, with a maximum magnification of 100x. This type of microscope might be used to study external features on an object or to examine structures not easily mounted onto flat slides.
