B. Subgroups
<span>When constructing a dichotomous key, the first step is to look at the group of objects or organisms and separate them into two groups based on a single distinguishing characteristic. Then continue to separate each of the groups until each object has its own separate set of characteristics.</span>
Answer:
A
Explanation:
The correct option would be A.
<em>According to the base-pairing rule, Adenine (A) always pairs with Thymine (T) (or Uracil in the case of RNA) while Guanine (G) always pairs with Cytosine (C).</em>
Hence;
A-U
G-C
A-U
A-U
C-G
T-A
Therefore, AGAACT would be transcribed to UCUUGA.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
A long high sea wave caused by an earthquake, submarine landslide, or other disturbance. Undersea earthquakes, which typically occur at boundaries between Earth's tectonic plates, cause the water above to be moved up or down. Tsunami waves are formed as the displaced water, which acts under the influence of gravity, attempts to find a stable position again.
<u>There are five groups of factors responsible for the kind, rate and extent of soil development. They are: Climate, organisms, parent material, topography and time. Soil from one place is different from another because of the differences in the influence of these factors.</u>
