Roche/454 pyrosequencing , useful for high throughput sequencing techniques. This method utilizes pyrosequncing which allows for sequencing as the sequence read out can be achieved at the same time as the sequence is extended.
High- throughput screening is the use of automated equipment to rapidly test thousand to millions of samples for the biological activities at the model organism, cellular, pathway or molecular level.Many DNA samples can be sequenced simultaneously.
Sanger DNA sequencing also known as chain termination. This method is used only for the single DNA fragment at a time. Amplicon sequencing with NGS is efficient and cost effective . It is required a DNA polymerase , nucleotide , dideoxynucleotides and buffer.
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The ecosystem requires a constant input of energy because energy cannot be stored. The enrgy always gets converted for supporting life in the ecosystem. Some amount of energy is also lost during this conversion. So if there is not a constant supply of energy, then the ecosystem will ultimately breakdown. In case of humans, the energy is received by consuming food. The energy of the food geets converted to the enrgy that helps a human being to complete his daily chores. So energy is being lost at every step. To regain this energy the humans have to eat food regularly. This way the humans need a constant supply of energy to survive. It is the same in the case of all living things in the ecosystem.
Answer:
water
Explanation:
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