Answer:
a. eukaryotes have three different RNA polymerases, whereas prokaryotes only have one RNA polymerase
Explanation:
The initiation represents the beginning of RNA production. Initially, RNA polymerase (transcription catalyzing enzyme) recognizes in DNA the so-called promoter regions. In prokaryotes, there is only one type of RNA polymerase; In eukaryotes, three RNA polymerases are described, and thus the transcription process in this group is more complex.
In the initiation step, we also observe the opening of the DNA strand, a process known as unfolding. This opening occurs because of the rupture between the connections that arise between the bases.
Answer:
e. None of the above
Explanation:
For me as a Researcher, the reason could be increased Concentration of your DNA sample which you are using as your template. Try to decrease the concentration of DNA (up to 100 ng per reaction is enough and can increase up to 200 ng). so the reason for getting non specific bands is increase concentration of DNA which results in non specific amplification and also degradation of DNA in the reaction which you can see in your gel electrophoresis results.
i always corrected my results using the same technique that is lowering the concentration of DNA between 100 and 200 ng per single reaction of PCR.
Gregor Mendel
Genetics as a scientific discipline stemmed from the work of Gregor Mendel in the middle of the 19th century.
Answer:
Ubiquitin ligase is a protein enzyme recruits Ubiquitin-conjugating enzyme that come with the ubiquitin and catalyzes the transfer of ubiquitin to the protein substrate of interest and destructed by the proteosome.
miRNA are small RNA molecules with no coding behavior that binds with the mRNA with the help of base pairing of complementary codes. It helps in mRNA degradation and cutting of poly-A tail of mRNA type of gene expressing.
A gene-specific transcriptional repressor helps in preventing the transcription process of the mRNA by inhibiting the binding of the RNA polymerase to the promoter.
