Question

The DNA sequence of the expression plasmid adjacent to the ribosome binding site is: ---GGATCCATCTGCGAATTC---- ---CCTAGGTAGACGCTTAAG--- It contains a BamH1 site (G^GATCC) and a EcoR1 site (G^AATTC). In order for the synthetic gene to be inserted between these two sites, it should be constructed to have which restriction sites at its beginning and at its end

Answer 1

Answer:

BamH1 (G^GATCC), followed by BamH1 (G^GATCC).

BamH1 (G^GATCC), followed by BglII (A^GATCT).

BglII (A^GATCT), followed by BglII (A^GATCT).

Explanation:

In order for the synthetic gene to be inserted between these two sites,  it should be constructed to have restriction sites

BamH1 (G^GATCC), followed by BamH1 (G^GATCC).

BamH1 (G^GATCC), followed by BglII (A^GATCT).

BglII (A^GATCT), followed by BglII (A^GATCT).

at its beginning and at its end

Artificial gene synthesis or gene synthesis, sometimes known as DNA printing is a method in synthetic biology that is used to create artificial genes in the laboratory. Based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that it does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size.