Ans.
Gene regulation or regulation of gene expression involves mechanisms, used by the cells to enhance or reduce the expression of specific genes to make proteins or RNA. Gene regulation occurs at transcriptional level and post-transcriptional level, which involves regulation at translational level or protein level.
Regulation at translational level or protein level is also important as regulation at transcriptional level. Translational regulation controls formation of proteins from mRNA molecules and includes non-coding mRNAs and repressor proteins. It is important for cell growth, differentiation and cellular response to stress and provides an immediate adjustment of gene expression by directly regulating the protein concentration.
Regulation at protein level involves regulation of active protein. It includes regulation by various small molecules, post-translational modifications (such as phosphorylation), and proteolysis. Regulation only at transcriptional level is not sufficient to provide proper gene regulation and leads to various drawbacks, such as Fragile X Syndrome (due to defect in a protein).
Thus, 'gene regulation is important both at transcriptional level and at post-transcriptional level (during translation or protein level).'
mRNA (Messenger RNA) is translated by ribosomes and contains the code that specifies the sequence of amino acids in polypeptide chain.
A single-stranded ribonucleic acid molecule is known as messenger RNA(mRNA) plays a major role in <u>translation</u>.
Translation is the method by which an mRNA codes for a certain protein. mRNA provides the template for<u> protein synthesis</u>.
The ribosome translates the mRNA that is produced from the DNA into a chain of certain amino acids and<u> protein synthesis</u> is facilitated by this <u>amino acid</u> sequence.
<u>The genetic code</u>, which connects the DNA sequence to the amino acid sequence of proteins, is used to "read" the mRNA. Each group of three nucleotides in mRNA forms a codon, and each codon corresponds to a particular amino acid (triplet code).
Thus mRNA contains the code that specifies the<u> sequence of amino acids</u> in a polypeptide chain.
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