Answer:
Explanation:
Hello!
The scientist created an experimental strain that produces a modified RNA polymerase with a single amino acid substitution. This mutation is supposed to change the elongation rate of the mRNA during transcription.
The dependent or response variable, is the one the researchers are interested in, meaning, are the characteristics that the researcher will pay attention to and measure during the experiment.
In this example, the researcher is interested in testing the max elongation rate during transcription, which is the dependent variable of this experiment.
In the second part of the experiment, both strains of yeast, wilds, and experimental, where exposed to 40ug/mL solution of amanitin and recorded the maximum elongation rate of the RNA. This is naturally to test the effects of amanitin over the elongation rate of the mRNA in both strains.
The control group is a set of experimental units that are exposed to the same conditions as the experimental groups, with the exception that they receive no treatment (or they receive a "no effective" treatment often called a placebo). The purpose of a control group is to know the natural response of the experimental units to a treatment-free environment, this way when comparing both groups, the researcher will be able to observe the differences or changes due to the applied treatments.
In the second experiment, there are missing two control groups, one made of the wild strain and the other made of the experimental strain, exposed to the same conditions as the treated strains.
I hope this helps!
