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3 years ago

A scientist wants to insert a new gene into a bacterium. What is the first step in this process?

Biology

2 answers:

In-s [12.5K]3 years ago

7 0

In an experiment, researchers first insert a piece of DNA like a gene, into a circular piece of DNA called a plasmid.

Lunna [17]3 years ago

3 0

Answer:

The correct answer is- cutting the gene out of DNA with enzyme.

Explanation:

To insert a gene into a bacteria first the gene of interest should be cut out of the DNA sequence by the help of an enzyme called restriction endonuclease. A specific restriction endonuclease cut the gene of interest by identifying restriction site and making cut at that particular restriction site.

Then this gene of interest is ligated in a plasmid vector which have its own origin of replication and a selection marker. Then this plasmid vector having the gene of interest is transferred or inserted into bacteria by methods like heat shock, gene gun, etc. After transformation, it will express proteins in bacteria according to the gene of interest it has.

So the first step in this process is cutting the gene out of DNA with enzyme.

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Please help option 1 option 2 option 3 option 4

zepelin [54]

I think option one because if the molecules are moving in place, they are tightly packed like a solid. if they are moving around each other, that would mean they could move more freely like a liquid.

4 0

3 years ago

Name a biotic factor in the room you are in right now.Name a biotic factor in the room you are in right now.

timama [110]

Answer:

mt brothers and sisters, dog, cat,

Explanation:

6 0

3 years ago

Which value is furthest from 0 on the number line? A. 20 B. -21 C. |20.5| D. |21.5|

Bond [772]

D is your answer :)

Hope this helps

6 0

4 years ago

Read 2 more answers

A vestigial-winged, hairy male is crossed with a wild-type female, homozygous at both genes. This F1 generation is crossed to pr

loris [4]

Answer:

The expected proportion of F2 individuals which are homozygous recessive at both genes is 1/16

All of the F1 generation will have the normal phenotypes for both genes

There will be no homozygous individuals in the F1 generation

Most of the F2 generation will have the normal phenotypes for both genes

Explanation:

A winged hairy male is crossed with a wild type female which is homozygous

Let the allele for vestigial trait be v

allele for wild trait be w

allele for hairy trait be h

Allele for non hairy be n

The genotype of parents would be

vvhh * wwnn

Thus in F1 generation all the offsrping will have normal phenotypes for both genes

In F2 generation

vwnh * vwnh

Out of 16 offspring only one wil be homozygous recessive i,e

wwnn

Rest all 15 will have normal phenotypes.

Hence, the correct answer is

The expected proportion of F2 individuals which are homozygous recessive at both genes is 1/16

All of the F1 generation will have the normal phenotypes for both genes

There will be no homozygous individuals in the F1 generation

Most of the F2 generation will have the normal phenotypes for both genes

8 0

4 years ago

Draw a pyramid of energy to show how energy flows through the antarctic food web. Use

GuDViN [60]

Answer:

The desired graph of the Antarctic food web is attached:

Explanation:

A food web shows the taking care of connections between the life forms in a specific biological system, fundamentally what eats what. It shows how vitality courses through the biological system. Creatures can be separated into producers, different level of consumers, and decomposers inside a food web:

producers in the Antarctic marine biological system are: ocean ice green growth, phytoplankton, macroalgae, microalgae

primary, second-level, third level, fourth level, and fifth-degree of consumers are straightforwardly or by implication rely upon producers as they structure a significant food hotspot for creatures that feed by grazing. Models incorporate snails, imps, and corals. These consumers thusly will be eaten by different creatures, for example, ocean stars. At the head of the food web, there are bigger consumers, for example, fish, penguins, and seals. energy moves from producers to the consumers and there is a large part of the energy loss during the process only 10% of energy gets to the subsequent trophic level.

3 0

4 years ago