Answer:
I don't understand thequestion
Answer:
Figures attatched
Explanation:
1. Restriction enzymes cut the DNA in specific places of a specific sequence. Each restriction enzyme has different sequences of recognition.
2. Palindromic sequences are sequences that can be read the same in both senses (3' and 5'), for that reason restriction enzymes can cut both DNA strands
Now, for the first image: if a molecule has 5 restriction sites that are known for a single restriction enzyme, the enzyme will cut the DNA 5 times. If you see the image, the number of fragments is the number of restriction sites +1. In this case, it is 5+1=6 DNA fragments.
In the case of two different palindromic sequences, two different restriction enzymes recognize different sequence. If just one enzyme is present, the only cutting site will be the one that has the palindromic sequence recognizable. So, the number of fragments will be 1+1=2 DNA fragments (figure 2)
Answer:
Extension
Explanation:
Polymerase chain reaction is given by Kary Mullis and it contains three steps denaturation, annealing, and extension. In denaturation, the temperature is raised to 96° c so that the DNA can denature.
In annealing, the temperature gets reduced to 55°c so that the primer can bind to the complementary sequence. In extension, Taq polymerase is used to extend the new strand.
This enzyme is isolated from thermophilic bacteria and the temperature here is raised to 72°c so that the enzyme can extend the primer and make the complementary strand. So the right answer is extension.
Answer:
occurring, awarded, or appearing after the death of the originator
Explanation: