Answer:
Background
During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.
Explanation:
Results
Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.
Meiosis and mitosis are both preceded by one round of DNA replication; however, meiosis includes two nuclear divisions. The four daughter cells resulting from meiosis are haploid and genetically distinct. The daughter cells resulting from mitosis are diploid and identical to the parent cell.
so the similarities are:
-ways for cells to divide
-same number of chromosome as the original cell
-both have the basic 5 phases
-both processes go through chromosome replication
-Meiosis II is similar to Mitosis
I hope this is helpful :)))
have a nice day
Answer:
The given statements have been rearranged in the following order to depict the flow of events that account for lipid digestion in the human body:
- Mechanical digestion by chewing breaks food down.
- During digestion, gastric lipases break down fat into fatty acids and triglyceride molecules.
- Gastric lipase digests some triglycerides.
- The pancreas secretes gastric lipase into the small intestine, where the majority of fat digestion occurs.
- Lipid digesting enzymes from the pancreas break down the triglycerides into two free fatty acids and a monoglyceride.
- Lingual lipase is secreted from the pancreas and breaks down triglycerides.
- With the aid of pancreatic lipase, fats are further broken down into free fatty acids and monoglycerides.
- Products of fat digestion are packaged into micelles and transported to the enterocytes.
The process is started in the mouth. Action of the teeth physically breaks down the food and increases it surface area that allows for better access to it for the enzymes to carry out mechanical digestion. Gastric lipases are those in the stomach, most of the lipid digestion takes place in the small intestine with the help of enzymes secreted by the pancreas and the small intestine itself. After digestion, the final products are taken up by the lymphatic system.
Hope that answers the question, have a great day!
Explanation:
Avg speed × total times = total distance
20×25=500
Since acceleration in the 1st interval is same as deceleration in the 3rd interval and deceleration is from the same velocity achieved after acceleration, t
1
=t
3
s
1
=
2
1
×5t
1
2
s
2
=(5t
1
)t
2
s
3
=(5t
1
)×t
3
−
2
1
×5×t
1
2
=(5t
1
)×t
1
−
2
1
×5×t
1
2
s
1
+s
2
+s
3
=5t
1
t
2
+5t
1
2
⇒5t
1
2
+5t
1
t
2
=500−−−−1
2t
1
+t
2
=25−−−−2
Solving (1 ) and (2) ⇒t
1
=5,t
2
=15sec
Answer:
When one phosphate group is removed by breaking a phosphoanhydride bond in a process called hydrolysis, energy is released, and ATP is converted to adenosine diphosphate (ADP). Likewise, energy is also released when a phosphate is removed from ADP to form adenosine monophosphate (AMP).
Explanation:
