Answer:
The correct order is dehydration, embed in wax, cut into sections, staining
Explanation:
There are certain proceedings needed to obtain stained sections of vegetable or animal tissues for their microscope observations.
These steps are:
- Obtention of the material: The tissue is cut to an adequate size.
- Fixation: When tissues are extracted from the organism, they suffer autolysis and putrefaction, so they need to be fixated in order to keep their cells in the best state possible. Fixation involves preserving the original morphological and molecular characteristics of the tissue. Fixation avoids autolysis, putrefaction, distortion, and retraction of cells and the tissue that could affect its volume or morphology.
- Dehydration. Once fixated, the fixator must be eliminated and the tissue is dehydrated by using a gradual series of solutions with alcohol in ascendant concentrations. Dehydration must be gradual to avoid tissue deformation.
- Inclusion. To obtain thin cuts that can be observed under the optic microscope, the tissues must be included in a consistent, firm substance, that might be either hydrophilic or hydrophobic. A hydrophobic medium is paraffin wax, that provides hardness and plasticity.
- Cut. The tissue included in wax must be cut in slides or sections thin enough to allow the diffusion and penetration of light. A microtome is used to perform these cuts. When using paraffine for tissue inclusion, the cuts are about 5 to 20 micrometers of thickness.
- Stain. Once the cuts are performed, paraffin wax must be eliminated. This can be done by using an organic solvent. Then the tissue must be stained. Hematoxylin and Eosin are the most common dyes. Animal tissues in general do not have any natural color, so they need to be stained to be observed.
Answer:
monomers of MONOSACCHARIDES
Explanation:
Polysaccharides are large molecules formed from chains of POLYMERS linked together by glyosidic bonds. <u>MONOMERS are small sub units that formed polymers, they are therefore the building block of a polysaccharides. The monomers of polysaccharides are called monosaccharid</u>es (1 sugar molecule.) when two of these are joined together they formed disaccharides (two sugars.)
Polysaccharides are fromed by joining together condensation, (loss of water molecules,) of mutiple monosaccharides units and the reversal of this to add water molecules to sepate them to monosaccharies is sugar Hydrolysis.
Example of polysaccharides are starch, glycogen cellulose
Example of monosaccharides are glucose, galactose.
Disaccharides are common table sugar, sucrose, maltose, lactose
Answer:
To maintain homeostasis, the control center responds to the changes in the stimulus received from the sensor by sending signals to effectors
It will be convergent i hoped this help you have a nice day <3