Which of the following lists has the steps involved in the whole genome shotgun approach to Sequencing in the correct order? 1.
Sequence assembly 2. Insertion of genomic fragments into cloning vector 3. Transformation of E. coli with cloning vector 4. Sequencing of cloned fragment ends 5. Isolation and shearing of genomic DNA
Whole genome shotgun sequencing is the method in the genetics that are used to sequence the whole DNA fragments. Different DNA sequencing methods are chemical sequencing and Sanger sequencing.
The different steps that are involved in the whole genome shotgun sequencing are as follows. The DNA is isolated and broken DNA with the help of the restriction enzymes. The genomic fragments are inserted into the cloning vector for its multiplication. These fragments are then transformed into the E.coli with the cloning vector. The sequence assembly is important step in the sequencing. Than the sequencing of the cloned fragments are done to find the sequence of the whole genome.
