Answer:
Extraction of genomic DNA
Explanation:
Resuspend the cells(any number), smaller numbers also work, in 10-20 ul of TE buffer or milli Q water. boil for 5-10 min in a waterbath, quick chill, short spin. use the supernatant as a template for PCR.
If cells are very less, u can directly add cells to the PCR reaction without enzyme,boil for 5-10 min in a waterbath, let it cool slowly, short spin. Add enzyme to the supernatant and start the reaction at extension step followed by normal PCR reaction steps.
<h2>J-shaped growth curve</h2>
Explanation:
- J- shaped growth curve A curve on a graph that records the circumstance wherein, in another condition, the population thickness of a living being increments quickly in an exponential or logarithmic structure, yet then stops unexpectedly as ecological opposition (for example regularity) or some other factor (for example the finish of the reproducing stage) unexpectedly gets compelling.
- Population numbers regularly show extraordinary variance, giving the trademark 'blast and bust' patterns of certain bugs, or the ones seen in algal sprouts.
- This kind of population development is named 'thickness autonomous' as the guideline of development rate isn't attached to the population thickness until the last accident
