Answer - B
Reason - Since the area is in the tropics there will be time to time heavy precipitation, which over time the decomposed nutrients will get washed down into a run off and depleted.
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Answer: Pollution, climate change, habitat loss, or invasive species
When a specimen is to be observed under the light microscope, scientists usually stain the specimens using different type of chemical stains depending on the type of specimen to be observed. Staining of specimen make the structures that are to be observed in the specimen very visible under the microscope, this make it easy for scientists to observe their structures.