Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
The Law of Superposition.
The Law of Superposition states that each layer is younger than the one beneath it.
Answer:
1 = A
2 = B
3 = B
Explanation:
1) Black holes occur when really big stars collapse on themselves. And, in order to get big, the star has to be an old one.
2) A black hole is something huge that swallows up everything, while a protostar is just the very beginning of a star.
3) Scientist can't truly know for sure, but they can infer about them due to the effects of the surrounding areas.
Answer:
The living organisms in an ecosystem can be divided into three categories: producers, consumers and decomposers. They are all important parts of an ecosystem. Producers are the green plants. They make their own food.
Explanation:
