Answer:
The correct answer is - polyploidy.
Explanation:
Speciation can be two types allopatric and sympatric speciation. Sympatric speciation is the process of speciation is developing the new species that diverged from the common ancestral species while the ancestral and developed species both can share the same place or geological area. The main cause of such speciation is due to the change in their genetic level more than environmental conditions.
In this case, the frog's species show differences in their ploidy level as well as their mating call but polyploidy is the most likely the difference that evolved first rather than females call for mating.
Thus, the correct answer is - polyploidy.
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Limited factors would mean that as the population in a community grows individual species would have to compete for those limited factors as the population reaches its carrying capacity. Also, the population as a whole would only be able to reach a certain number before falling due to there not being enough of the limited factors (ex. food) for that population to continue growing exponentially.
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