This is because there might not be one restriction site bordering the gene to be cloned and the identical restriction site in the plasmid. Since these two restriction enzymes make compatible sticky ends, the insert has a chance of combining with the plasmid. To ensure efficient digestion, the two recognition sites should be more than 10 base pairs apart. If one of the enzymes is a poor cutter or if the sites are separated 10 base pairs or less, the digestions <span>should be performed sequentially</span>
Answer:
B. refraction
Explanation: hope this helps!
Answer:
<h2>C. What is the length of the S phase of the cell cycle?
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Explanation:
- Such type of technique that is used to tack or identified the passage or location of the metabolic reaction, organelles and some other during the research or experiment is known as radiolabelling.
- During the study of the synthesis of the DNA, the nucleotides are labeled to check the S phase of the cell cycle.
- Since the synthesis of the DNA takes place during this phase so the time and length of the S phase can be easily obtained during the experiment.
- This technique is also used for different purposes during the biomolecular experiment and some other disciplines.
Answer & explanation:
Amylase is part of enzymes, a group of large peptide molecules (formed by amino acids) whose role is to catalyze reactions in order to facilitate the synthesis of other biological molecules.
Amylase is found mainly in saliva (in the form of salivary amylase, or ptialin), acting in the breakdown of starch and glycogen in foods, reducing them to smaller particles, facilitating their digestion and absorption.
The action of enzymes depends on certain specific conditions, called optimal conditions. In the case of <u>amylase</u>, it depends on an optimum pH of 7 (neutral) and an optimum temperature of approximately 37 ° C.
This enzyme can still act between 35 ° C and 40 ° C, but below 35 ° C it is inactivated, preventing its functions from being performed, and above 40 ° C it suffers denaturation, causing changes in its structures.
Thus, it is concluded that the <u>temperature</u> (under optimal conditions) is important for enzymes because it keeps their actions and structures in proper operation.
Factories I think ? I don't know and nuclear plants
