Question

Concerning the Tools of Genetics Box Analysis of Cell-Cycle Mutants in Yeast: a. Describe how you would use replica plating of mutagenized, haploid yeast cells to identify temperature-sensitive (ts) mutations in essential genes needed for yeast growth and survival. b. Among the many ts mutations you found in part (a), how would you distinguish mutations in genes needed for cell-cycle progression from those in genes needed for other aspects of the life of yeasts

Answer 1

Genetic control of the cell division cycle in Yeast is given below.

 

Explanation:

• The genetics is the branch of biology that deals with the study of genes, genetic variation, and heredity in organism. Yeast cell is very useful to identify the genes that control cell division.
• A master plate is prepared with colonies that can grow only at the low and permissive temperature.
• The satin cloth is pressed on the master plate, and then that cloth is pressed on another replica plate. This replica plate is incubated at a higher temperature than the permissive temperature.
• After incubation, both plates will be compared. The colonies that will not appear on the replica plate but will be appearing on the master plate will have the temperature-sensitive mutation...