I believe the answer to this question is (D) habitat fragment. Habitat fragmentation is the process in which the loss of habitat causes a division in a larger habitat, resulting in smaller areas that are isolated from each other. In this the tigers are separated from other inhabitable nearby areas.
It has no effect. Density of a substance is the same no matter the size or shape of the sample.
Answer:
When the bladder is completely deflated, the fish has minimum volume and sinks to the ocean floor