Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
They did it to get more detailed pictures of space
Answer:
Un virus no es una célula sino un organismo microscópico que invade las células de sus huéspedes.
Explanation:
Answer:
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Answer:
c) It introduces a premature stop codon into the mRNA
Explanation:
Nonsense mutation is a type of point mutation (single nucleotide base is changed) which leads to premature stop codon. Stop codons are also called nonsense codons and that is way this type of mutation is called nonsense mutation. As a consequence, synthesized protein is incomplete and shorter than it should be (truncated protein), usually nonfunctional.
