Answer:
The three methods are: 1) Using H-Ras- SREBP- 2 Fusion proteins, the NH2 segment can be released by two sequentia
Explanation:
Sterol regulatory element binding proteins (SREBPs) are transcription factors attached to the endoplasmic reticulum. The NH2-segment, which activates transcription, is connected to membranes by a hairpin anchor formed by two transmembrane sequences and a short lumenal loop. Using H-Ras–SREBP-2 fusion proteins, we show that the NH2-segment is released from membranes by two sequential cleavages. The first, regulated by sterols, occurs in the lumenal loop. The second, not regulated by sterols, occurs within the first transmembrane domain. The liberated NH2-segment enters the nucleus and activates genes controlling cholesterol synthesis and uptake. Certain mutant Chinese hamster ovary cells are auxotrophic for cholesterol because they fail to carry out the second cleavage; the NH2-segment remains membrane-bound and transcription is not activated.
Answer:
The humanistic approach emphasizes the personal worth of the individual, the centrality of human values, and the creative, active nature of human beings. The approach is optimistic and focuses on the noble human capacity to overcome hardship, pain and despair.
Answer:
https://www.you tube.com/watch?v=RCjNN6JfSmE
This video gives stretches for the soleus muscle.
It's titled Soleus Stretch - Ask Doctor Jo, if you don't trust the link I answered. (Which I would get, I usually don't trust links from complete strangers on the internet).
Explanation:
Extrinsic muscles are contained within a region such as the hand or the leg, and the intrinsic muscles move the fingers but are found outside the region.
Explanation:

At the heart of it, the main difference is that a product business sells physical, tangible objects, whereas a service business provides value through intangible skills, expertise and time.