calcium is not required in the process of glycerinated method because glycerination process intrrupt the troponin- tropomyosin complex due to this interruption the bindings are exposed. So the glycerin makes the membrane suitable for the small molecules including ATP. Addition of ATP is highly required by the glycerinated muscle so that the myosin head actually binds the binding site so the ATP can easily available so we do not need the calcium for glycerinated muscle contraction
