- 20 cycles of PCR is necessary for the amplification target sequence
Mass is proportional to sequence length.
Let one base pair have mass M.
Thus,
will have mass 
And the 3.2kb target will have a mass of
For the total amount of amplified target sequence to constitute 50% (by mass) of the total DNA in the reaction mixture, it has to be amplified to such an extent that the total mass of the amplicon matter is also 
PCR amplification happens geometrically, doubling the DNA target with each cycle.
Thus, if the number of cycles in N,
Then,

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Answer:
Between two and four years
Explanation:
Sorry if Im wrong.
Because the client has been diagnosed with hyperthyroidism. The nurse expects him to exhibit the following clinical manifestations:
- Nervousness
- Increased appetite
<h3>What is associated with hyperthyroidism?</h3>
A hyperthyroidism means the overactivity of the thyroid gland that results in a rapid heartbeat and an increased rate of metabolism.
Generally, a nervousness is associated with hyperthyroidism because of central nervous system irritation. The appetite also increases with hyperthyroidism because of the increase in the metabolic rate.
Therefore, the nurse expects him to exhibit the following clinical manifestations that includes nervousness and Increased appetite.
Read more about hyperthyroidism
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