Oxygen.
Oxygen was fatal when organisms started using photosynthesis, this event was known as the Great Oxidation Event.
In eukaryotes, there are two distinct types of cell division: a vegetative division, whereby each daughter cell is genetically identical to the parent cell (mitosis) and a reproductive cell division, whereby the number of chromosomes in the daughter cells is reduced by half to produce Haploid gametes (meiosis)
Answer:
-use PCR to amplify the gene for GFP
-perform a restriction digestion of the GFP gene and the plasmid
-ligate together the GFP gene and the plasmid to generate a recombinant plasmid
-transform bacteria with the recombinant plasmid using electroporation
-plate the bacterial cells, and screen for positive transformants