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Licemer1 [7]
3 years ago
8

Why choose that gene for PCR in the bacterial identification lab?

Biology
1 answer:
Reika [66]3 years ago
3 0

Answer:

Background

During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.

Explanation:

Results

Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.

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Explanation:

We have attached a figure that shows the diagram of the given equation.

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In your biology class, there was a discussion about climate change. You learned that human activities are changing the compositi
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Answer:

Below

Explanation:

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Let's break it down shall we:

When a human breathes, they release 35,000 to 50,000 ppm (parts per million) of CO2 with every exhale. On average, we breathe out 20,000 times a day. Multiply that by the amount of people on Earth, about 7.8 billion, you get 1.716x10^14 breaths a day. Therefore, you have that times 50,000 ppm of CO2 released. That sure is a lot, however, let's look at how much carbon dioxide is released from coal alone in a day. In general, the world consumes over 8.5 million tons of coal a year, and in the US alone 14,000 tons are burnt in one day. When you burn one pound of coal, around 2 pounds of CO2 is released, as the carbon of the coal mixes with the oxygen requiring it to burn. Therefore, 28,000 tons of CO2 is put into the environment from coal alone (not even including all of the other fossil fuels like natural gas and oil), showing how extensive the CO2 burning of fossil fuels is.

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