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Licemer1 [7]
3 years ago
8

Why choose that gene for PCR in the bacterial identification lab?

Biology
1 answer:
Reika [66]3 years ago
3 0

Answer:

Background

During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.

Explanation:

Results

Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.

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What is a complex sugar
storchak [24]

Answer:

Complex Carbohydrates: Also know as polysaccharides (poly = many), these carbohydrates have more than two units of glucose (sugar) linked together. Polysaccharides are generally not sweet and are not water soluble. Starches and dietary fiber are complex carbohydrates.

Complex carbs pack in more nutrients than simple carbs. They're higher in fiber and digest more slowly. This also makes them more filling, which means they're a good option for weight control. They're also ideal for people with type 2 diabetes because they help manage blood sugar spikes after meals.

Simple carbs — also known as simple sugars — contain one or two sugar molecules, whereas complex carbs have three or more. A simple sugar may be a mono- or disaccharide.

7 0
3 years ago
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Question 11 of 25
kicyunya [14]
B, production of waste
7 0
3 years ago
Which most accurately describes the basis of the scientific method?
Tanzania [10]

The scientific method's foundation is best described as "the process of hypothesis and testing through which scientific inquiry happens." Thus, the correct option is D.

<h3>What is scientific method?</h3>

The scientific method is a set of actions that are followed in order to develop scientific knowledge.

The scientific process includes the following steps:

  1. To make observations on a particular natural occurrence and then generate questions based on those observations.
  2. To answer these questions, you must propose a credible explanation (i.e., a hypothesis).
  3. To put the working hypothesis to the test using experimental and/or observational methods.
  4. To gather information in order to confirm or refute a working theory.
  5. Analyze the findings and develop conclusions.

For more information regarding scientific method, visit:

brainly.com/question/17216882

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5 0
2 years ago
Paclitaxel affects not only cancer cells, but normal cells as well. Would the effects of Paclitaxel be seen first in organs that
Svetlanka [38]

Answer: The most affected would be organs that have QUICKLY dividing cells (like the intestine and hair follicles).

Explanation:

Cancer cells are cells that divides uncontrollably giving rise to a mass of tissue called tumour. They grow faster than a normal cell in an uncoordinated manner, and continues to grow after the initial stimulus has ceased.

Paclitaxel is a drug that is approved for the treatment of cancer affecting different parts of the body. It's a microtubule-stabilizing drug whose mechanism of action is to induce mitotic arrest in the cancer cells.

Paclitaxel in its cause of action not only affect cancer cells but normal cells as well. To justify this statement, as stated earlier, the mechanism of action of paclitaxel is to induce mitotic arrest. Therefore the cells of organs where rapid mitosis occurs would be most affected. Skin cells, hair follicles and the cells lining our intestines (epithelial cells) all have high rates of mitosis as these tissues constantly need to be replaced.

5 0
2 years ago
How much magma is formed at a transform boundary<br> i need this asap
saul85 [17]

Answer:

magma doesnt form at transform boundaries, but it does form at divergent and convergent bounderies.

Explanation:

The divergent boundaries pull apart from each other creating a weak spot in the crust, allowing magma o come through and reach the surface. Some of the rock above the subducting plate melts and forms magma. Because the magma is less dense than the surrounding rock, it rises to the surface.

At convergent boundaries magma is formed where water from a subducting plate acts as a flux to lower the melting temperature of the adjacent mantle rock

i hope this helps a little bit (:

7 0
2 years ago
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