1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Volgvan
3 years ago
11

g A team of researchers suspects that RegX may play a role in regulating cytoskeletal reorganization by activating KinY. To test

whether these two proteins can interact, they conducted two variations of the following experiment: 1) Transform mouse cells with expression plasmids encoding the proteins of interest. 2) Express proteins of interest inside cells. 3) Lyse cells 4) Conduct a Co-IP on the cell lysate against GST or GST-tagged KinY (depending on the specific experiment). 5) Run western blots to see which proteins are present in the immunoprecipitates. The results _________________________________________.
Biology
1 answer:
andriy [413]3 years ago
3 0

Answer:

It is expected to observe the immunoprecipitation of the GST-tagged KinY complex, thereby indicating the role of RegX in the mechanism of cytoskeletal reorganization.

Expanation:

Western blot is a technique widely used in molecular biology laboratories in order to identify proteins and also to quantify their expression levels. This technique is useful to detect proteins with high specificity, in this case the technique has been used for the detection of an immunoprecipitated (i.e., GST-tagged KinY complex) in order to confirm the role of the upstream protein RegX in cytoskeletal organization.

You might be interested in
Komodo dragons are a species endemic to which country?.
nadezda [96]

Answer:

Indonesian

Explanation:

Indonesian is named after the island where humans spotted a dragon-like creature for the first time, the Komodo dragon is an endemic Indonesian species found on Komodo Island and its neighboring islands.

5 0
2 years ago
Which substance is an inorganic molecule?
Jobisdone [24]
Compounds that do not contain carbon
7 0
3 years ago
Read 2 more answers
If the circulatory system failed what would happen to the heart rate?
Gelneren [198K]
The heart rate would decrease because there is less oxygen being supplied to the cells for cellular respiration, and also your muscles won't get the blood it needs. Blood also contains oxygen and glucose which cells need to convert food to energy and to survive, so if the cells don't get blood they will die which will eventually kill the organism
8 0
3 years ago
Read 2 more answers
based on your observation, you suggest that the presence of water could accelerate the growth of bread mold
Marta_Voda [28]
Molds and fungi are found everywhere inside and outside, and can grow on almost any substance when moisture is present. Molds when they reproduce make spores, which can be carried by air currents. When these spores land on a moist surface that is suitable for life, they begin to grow. Molds are essential to the natural breakdown of organic materials in the environment. Mold is normally found indoors at levels that do not affect most healthy individuals. When these levels become abnormally high as determined by indoor air quality testing or a mold inspection, remediation is recommended to be carried out by a professional remediation company.
6 0
3 years ago
Genetic codes contain _____.
denis23 [38]
<em>Genetic codes contains <u>ORGANISMS.</u></em>
6 0
3 years ago
Read 2 more answers
Other questions:
  • The sodium-potassium pump establishes concentration gradients:
    5·1 answer
  • Here’s another free question! No work involved &lt;3
    5·1 answer
  • The term evolution means
    7·2 answers
  • How can biotic and abiotic factors affect the size of a population? Give an example of a biotic and an abiotic factor that could
    8·2 answers
  • Why does an airplane's weight change during the<br>course of a flight?​
    6·2 answers
  • Tell some thing about Ch 9 of class 8th ​
    11·1 answer
  • Identify how many carbons from one glucose molecule enter one round of the Krebs cycle
    7·1 answer
  • What elements are found in carbohydrates
    13·1 answer
  • What is the arrangement of oxygen molecules in a liquid state
    8·1 answer
  • Explain what the relationship is between predators and scavengers in the African savanna.​
    11·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!