Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Amoeba: Also ameba. A single-celled (protozoan) organism that constantly changes shape. The word "ameba" is from the Greek "amoibe" meaning "change." Ameba can infect the bowels to cause diarrhea and the liver to cause abscess formation. Here is more information. The definition of an amoeba is a one-celled organism, common in water and soil, possessing no set cell organs, structure, or defining shape. An example of an amoeba is an invisible organism called Entamueba histolytica which is found in tropical areas that are unclean, and causes the deadly disease dysentery. Hope this helps! Mark brainly please!
Answer:
Herbivores, Carnivores, Omnivores
plz mark me as brainliest.
C. Condensation and Evaporation are the two ways in which energy is transferred between layers of the atmosphere.
