A change in their food, from trees to grass in the Great Plains.
We use Chargaff's rule to get the answer.
[A] + [G] = [C]+ [T].
[A] + [G] + [C] + [T] = 100%
Where is A is Adenine, G is Gaunine, T is Thymine and C is Cytosine.
In DNA, Adenine always pairs with Thymine, and Guanine always pairs with Cytosine.
Therefore if Thymine is 35%, then Adenine will also be 35% to make 70% in total.
The remaining percentage will be 100% - 70% = 30%.
The 30% will be shared equally among Cytosine and Guanine, at 15% each. Therefore Cytosine will be 15%
The stem of the plant should be cut at least three to four centimeters above the surface level.
The stem should be tightly fixed into the glass capillary.
All connection should be made air-tight by using paraffin.
Initial and final readings should be carefully noted.