The primary structure of a protein - its amino acid sequence - drives the folding and intermolecular bonding of the linear amino acid chain,which ultimately determines the protein 's unique three-dimensional shape.
When the beta-globin gene is inserted into the bacterial chromosome, during insertion, beta-globin forms a stop codon between the coding sequences of beta globin, resulting in a reduced amino peptide. Acid on translation
Furthermore beta-globins form a tertiary protein complex with alpha-globin, which in addition to the ham-subgroup forms functional hemoglobin.
Since we only put the beta-globin gene on the bacterium-chromosome that is not enough to make a functional protein, we found a non-functional protein
When a child is taken to the hospital they are out of their regular routine and are some place unfamiliar . They are not familiar with anything so a familiar object may calm their nerves and give them a since of security
