this mutation may change the open reading frame of the resulting RNA sequence and its final product, which is a protein in the case that this gene is used to synthesize a messenger RNA (mRNA) sequence
Explanation:
During the transcription, a region of DNA named 'gene' is used as template to produce an RNA molecule, typically a primary transcript of mRNA (pre-mRNA). Subsequently, this pre-mRNA suffers a process named RNA processing in order to generate a mature mRNA which is finally used to create a protein by a process called translation. If a deletion occurs during transcription, it may change the open reading frame (ORF) of the resulting mRNA when the mutation occurs in an exon of the protein-coding gene (i.e., occurs a frameshift mutation), while this deletion may not have any effect if it is localized within the introns which are removed during RNA processing. A frameshift mutation will change the amino acids that are added to the nascent polypeptide chain during translation.
The material that are used by biotechnologists would be C. Restriction endomuclease. These are specialized group of enzymes that can cleave or break multiple phosphodiester bonds between DNA sequences.
