Question

Protein Omicron contains 4 subunits. Two subunits are 65 kDa in size and are held together by a disulfide bond. One subunit is 82 kDa and the last subunit is 45 kDa. You forget to add beta-mercaptoethanol to your protein sample before loading the sample on the polyacrylamide gel. What size bands would you see

Answer 1

Answer:

The correct answer is - 130 kDa, 82 kDa, 45 KDa.

Explanation:

In the question given is there are 4 subunits in protein omicron in which two subunits are - 65 kDa and the other two subunits are - 85 kDa and 45 kDa.

So the size band would be in absence of beta-mercaptoethanol which is very essential to break down disulfide bonds that held together these subunits:

Size band = 65 KDa + 65 KDa

= 130 KDa  (one band) ( 65+65=130),

82, 45 as without beta mercaptoethanol disulfide bond is not broken .

Thus, the correct answer is - 130 kDa, 82 kDa, 45 KDa.