Identify the plasmid that must contain the CD3γ core promoter sequence but the fewest or no negative regulatory sequences. Based
on the data in Figure 2, describe the most likely cause of the variation in luciferase activity among the cells that contain plasmids pCD3γ-419 , pCD3γ-309 , pCD3γ-239 , and pCD3γ-199 . Calculate the approximate percent increase in luciferase activity between cells containing plasmid pCD3γ-59 and cells containing plasmid pCD3γ-149 . Round to the nearest whole number.
The luciferase mRNA is translated to its corresponding protein in the ribosome which is present in the cytoplasm of the cell.
In the cytoplasm, the chain of ribosome sits on the mRNA and forms a structure called a polysome. This helps in the fast synthesis of protein.
The three-nucleotide genetic code in the mRNA which is being read by the ribosome dictates the correct arrangement of amino acids through particular tRNA.
